Compact Culture System™ (CCS)
Live zooplankton such as rotifers and Artemia are essential first-feeds for the larve of many fish (for example, Clownfish). The APBreed Compact Culture System (CCS) is designed to make benchtop-scale culture of zooplankton easy and reliable.
The CCS combines a conveniently-sized 5-gallon bucket with a custom-made fitting that incorporates an aerator and a particulate waste trap. Aeration provides oxygenation and water circulation that draws particulate wastes into the trap (floss).
Daily maintenance of the culture (aside from feeding and harvesting) requires only 5 minutes to flush the wastes from the trap and brush the interior surfaces of the bucket. The CCS is sturdy and easily transported, and includes a flexible translucent cover that isolates the culture from contamination.
CCS Starter Kit
Our CCS Starter Kit includes the CCS BASIC (see below) along with an FDA approved 5 gallon bucket with sticker showing liter volume graduations
CCS Basic Set
Our CCS Basic Set includes specialized parts required to turn your own 5 gallon bucket into a continuous growth and harvest high-density rotifer system. The package includes:
- Clips (2)
- Arms (2)
- T-fitting (1)
- Bucket Lid (1)
- Descender Pipe (1)
- Floss Support Tube + Clamp (1)
- Floss ( 1)
- Floss Retainer (1)
- Rigid Airline (1)
- Air Diffuser (1)
You will need to supply...
- 5 Gallon Bucket *
* Included in CSS Starter Kit
- Commercial performance at bench top scale
- Easy to use – easy maintenance
- High-density cultures
- Stable cultures
- Optimized oxygenation
- Optimized circulation
- Optimized waste control
- Simple, durable design
- Easily transported
- FDA approved bucket
- Includes cover
- Remove Airstone
Pull airstone off the rigid airline.
- Insert Rigid Airline into T-Assembly
Push airline through the hole in the top of the T-fitting.
- Attach Standpipe
Insert top of floss carrier standpipe into T-fitting.
- Re-attach Airstone
Carefully slide airstone on to the end of the rigid airline.
- Attach Clips to Bucket
Insert tab of clip under rim of bucket, swing tab into place against inside of bucket. Attach 2nd clip on opposite side of bucket.
- Fitting T-Assembly into Bucket
Seat one arm of the T into a clip, push while holding onto opposite clip to deform rim of bucket and seat other arm in opposite clip.
- Add 14 Liters Saltwater
Add 14 liters of saltwater, rotifer feed, and rotifer starter culture.
- Attach Air Source to Top of Rigid Airline
Seat bucket cover in place; stretch slightly to fit over clips. Attach tubing from air source to the rigid airline.
- Harvest Culture & Water Change
Once culture reaches desired density, harvest 20-30% each day.
- Clean Rotifer Floss
Deform bucket rim to release T from clips, lift T out of water and let free water drip into bucket. Flush waste from floss with strong spray of water.
- Brush Bucket Surfaces
Brush interior surfaces of the bucket to remove adhering detritus, so it can be trapped and removed by the floss. Replace harvested volume with new saltwater.
- Feed Rotifer Culture
Re-install T into bucket clips, feed culture, re-attach cover, re-attach air supply.
Harvesting the Rotifer Culture
- It is best to harvest rotifers every day
- Approximately 30% is a good daily harvest rate
- If one day you need more rotifers, it is OK to harvest more heavily. To increase the number of rotifers harvested every day, increase the feed rate but keep a consistent harvest rate. For fewer rotifers just reduce the feed rate.
- To harvest rotifers, remove the appropriate volume of culture. The rotifers can be concentrated on a screen and collected, or you can simply pour the rotifer culture water into your tank.
- Replenish the rotifer culture with new saltwater.
- Important: Stir culture first to ensure a representative sample
- Pipet a sample (typically 10 mL) into a vial. To immobilize the rotifers for counting, add 5 drops of vinegar and mix.
- Important: Stir sample so it is well-mixed and then quickly — before rotifers have time to settle out — use a pipet to transfer a known volume to a counting slide.
- Count the rotifers in that volume and calculate the number per mL or L.
- Your count should include at least 100 rotifers to be statistically reliable. If it is lower, count additional volumes.
- The most accurate counts are made with a Sedgewick-Rafter type slide that has a grid of 50 x 20 = 1,000 squares. You can count the rotifers in 100 squares and multiply by 10 to get the number of rotifers/mL. If the number is below 100, count more squares; if you count 200 squares, multiply by 5.
CCS Rotifer Culture Specifications
- Air flow: Minimum 1.5 - 2 LPM
- Salinity: 10-35 ppt (S.G. 1.0075 - 1.026), optimum 15-20 ppt
- Temperature: 27°C/80°F (L rotifer type); 30°C/86°F (S rotifer type)
- pH: 7-8 is optimal, 6.7 min, 8.5 max
- Ammonia: Maximum 1 mg/L NH3
- Suggested Feed: RGcomplete
- Feed Rate: 11 mL per million rotifers to be harvested
- Optimal Feedings per Day: "Continuous" feeding (~ every 3 hours)
- Minimum Feedings per Day: at least 2x per day for good production
- Harverst Rate: 20 to 30% Daily