Compact Culture System (CCS)

Live zooplankton such as rotifers and Artemia are essential first-feeds for the larve of many fish (for example, Clownfish). The APBreed Compact Culture System (CCS) is designed to make benchtop-scale culture of zooplankton easy and reliable.

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The CCS combines a conveniently-sized 5-gallon bucket with a custom-made fitting that incorporates an aerator and a particulate waste trap. Aeration provides oxygenation and water circulation that draws particulate wastes into the trap (floss).

Daily maintenance of the culture (aside from feeding and harvesting) requires only 5 minutes to flush the wastes from the trap and brush the interior surfaces of the bucket. The CCS is sturdy and easily transported, and includes a flexible translucent cover that isolates the culture from contamination.

CCS Starter Kit

Our CCS Starter Kit includes the CCS BASIC (see below) along with an FDA approved 5 gallon bucket with sticker showing liter volume graduations

CCS Basic Set

Our CCS Basic Set includes specialized parts required to turn your own 5 gallon bucket into a continuous growth and harvest high-density rotifer system. The package includes:

  • Clips (2)
  • Arms (2)
  • T-fitting (1)
  • Bucket Lid (1)
  • Descender Pipe (1)
  • Floss Support Tube + Clamp (1)
  • Floss ( 1)
  • Floss Retainer (1)
  • Rigid Airline (1)
  • Air Diffuser (1)

You will need to supply...

* Included in CSS Starter Kit

For more information about working with rotifers, see our rotifer support pages.

  • Commercial performance at bench top scale
  • Easy to use – easy maintenance
  • High-density cultures
  • Stable cultures
  • Optimized oxygenation
  • Optimized circulation
  • Optimized waste control
  • Simple, durable design
  • Easily transported
  • FDA approved bucket
  • Includes cover
  • Economical
  1. Remove Airstone
    Pull airstone off the rigid airline.
  2. Insert Rigid Airline into T-Assembly
    Push airline through the hole in the top of the T-fitting.
  3. Attach Standpipe
    Insert top of floss carrier standpipe into T-fitting.
  4. Re-attach Airstone
    Carefully slide airstone on to the end of the rigid airline.
  1. Attach Clips to Bucket
    Insert tab of clip under rim of bucket, swing tab into place against inside of bucket. Attach 2nd clip on opposite side of bucket.
  2. Fitting T-Assembly into Bucket
    Seat one arm of the T into a clip, push while holding onto opposite clip to deform rim of bucket and seat other arm in opposite clip.
  3. Add 14 Liters Saltwater
    Add 14 liters of saltwater, rotifer feed, and rotifer starter culture.
  4. Attach Air Source to Top of Rigid Airline
    Seat bucket cover in place; stretch slightly to fit over clips. Attach tubing from air source to the rigid airline.
  1. Harvest Culture & Water Change
    Once culture reaches desired density, harvest 20-30% each day.
  2. Clean Rotifer Floss
    Deform bucket rim to release T from clips, lift T out of water and let free water drip into bucket. Flush waste from floss with strong spray of water.
  3. Brush Bucket Surfaces
    Brush interior surfaces of the bucket to remove adhering detritus, so it can be trapped and removed by the floss. Replace harvested volume with new saltwater.
  4. Feed Rotifer Culture
    Re-install T into bucket clips, feed culture, re-attach cover, re-attach air supply.

Harvesting the Rotifer Culture

  1. It is best to harvest rotifers every day
  2. Approximately 30% is a good daily harvest rate
  3. If one day you need more rotifers, it is OK to harvest more heavily. To increase the number of rotifers harvested every day, increase the feed rate but keep a consistent harvest rate. For fewer rotifers just reduce the feed rate.
  4. To harvest rotifers, remove the appropriate volume of culture. The rotifers can be concentrated on a screen and collected, or you can simply pour the rotifer culture water into your tank.
  5. Replenish the rotifer culture with new saltwater.

Counting Rotifers

  1. Important: Stir culture first to ensure a representative sample
  2. Pipet a sample (typically 10 mL) into a vial. To immobilize the rotifers for counting, add 5 drops of vinegar and mix.
  3. Important: Stir sample so it is well-mixed and then quickly — before rotifers have time to settle out — use a pipet to transfer a known volume to a counting slide.
  4. Count the rotifers in that volume and calculate the number per mL or L.
  5. Your count should include at least 100 rotifers to be statistically reliable. If it is lower, count additional volumes.
  6. The most accurate counts are made with a Sedgewick-Rafter type slide that has a grid of 50 x 20 = 1,000 squares. You can count the rotifers in 100 squares and multiply by 10 to get the number of rotifers/mL. If the number is below 100, count more squares; if you count 200 squares, multiply by 5.

CCS Rotifer Culture Specifications

  • Air flow: Minimum 1.5 - 2 LPM
  • Salinity: 10-35 ppt (S.G. 1.0075 - 1.026), optimum 15-20 ppt
  • Temperature: 27°C/80°F (L rotifer type); 30°C/86°F (S rotifer type)
  • pH: 7-8 is optimal, 6.7 min, 8.5 max
  • Ammonia: Maximum 1 mg/L NH3
  • Suggested Feed: RGcomplete
  • Feed Rate: 11 mL per million rotifers to be harvested
  • Optimal Feedings per Day: "Continuous" feeding (~ every 3 hours)
  • Minimum Feedings per Day: at least 2x per day for good production
  • Harverst Rate: 20 to 30% Daily